The action epic screened in 4,252 domestic theatres for a $35,540 per-theatre average, and played in 8,900 overseas venues.
It opened number one in all its 107 countries, setting all-time opening records in 29 including the US, South Korea, Japan, Italy, Mexico, China, and Brazil.
Apart from the record global, international and domestic launches, the release set a new biggest single worldwide day mark of $117.6m on Saturday May 5.
On the domestic front, Spidey registered the biggest single opening day of $59.8m on Friday May 4, and a new record for the biggest gross in IMAX theatres of $4.8m from 84.
'This weekend, more than 80% of the moviegoers around the world chose Spider-Man 3 and we couldn't be more overwhelmed or elated by the global reception to this movie,' Jeff Blake, chairman of worldwide marketing and distribution for the Columbia TriStar Motion Picture Group and vice chairman of Sony Pictures Entertainment, said.
'We have always strived to deliver films with great stories that would excite and entertain audiences and credit for that goes first and foremost to our film-makers and cast: director Sam Raimi, our partners at Marvel, our producers Laura Ziskin, Avi Arad, and Grant Curtis, and our stars Tobey Maguire, Kirsten Dunst, James Franco, Thomas Haden Church, and Topher Grace.
'Spider-Man 3 builds on the first two films and satisfies the extraordinary expectations of fans everywhere. This was truly a massive achievement not only for Sony Pictures, but the film industry as a whole. Summer is finally here and we expect that moviegoers everywhere will find that the 2007 season will be one to remember.'