Lee and his cinematographer Matthew Libatique spent a day with the LA Lakers' basketball star Kobe Bryant during last year's playoffs.
The picture features the first score ever written by Bruce Hornsby and the original song Levitate. Kobe Doin' Work will be broadcast on May 16 on ESPN/ESPNHD.
Tribeca Film Festival executive director Nancy Schafer said, 'The Tribeca/ESPN Sports Film Festival has become an eagerly anticipated staple of the Festival and we look forward to showing films that embody the passion and spirit of sports.'
This is the third year of the Tribeca/ESPN Sports Film Festival and previously announced titles in the programme include Carlos Cuaron's football tale Rudo Y Cursi, Lost Son Of Havana about Cuban pitcher Luis Tiant's return to Cuba, Eric Bana's Love The Beast about his lifelong obsession with cars, and the NASCAR Little League picture Racing Dreams.
Tribeca runs from April 22-May 3.