Paul Sandberg and Marc Abraham will produce with Strike Entertainment's Eric Newman.
The Sigma Protocol was the last complete book that Ludlum, whose Jason Bourne novels Universal adapted into a $950m global franchise, finished before he died and was published posthumously in 2002.
The novel revolves around the son of a Holocaust survivor who gets sucked in to a military technology conspiracy.
Universal Pictures' senior vice president of production Scott Bernstein and creative executive David Ortiz will oversee the project for the studio.
Marcum and Holloway wrote Lionsgate's upcoming action sequel Punisher: War Zone and are attached to Summit Entertainment's Highlander adaptation. They are represented by ICM and attorney Mark Bisgeier.
Ludlum's works are an attractive proposition to Hollywood. In April it emerged that Denzel Washington is attached to The Matarese Circle, a cat-and-mouse tale of a US spy who must team up with his Russian nemesis.