Heading into Comic-Con the studio announced the next film from the world of Spider-Man will open on November 11, 2016.
The man charged with bringing to life the story of the supervillain alliance is writer-director Drew Goddard.
Avi Arad and Matt Tolmach are the producers. The Sinister Six comprises Doctor Octopus, The Vulture, Raven The Hunter, Electro, Sandman and Mysterio.
Columbia Pictures will then release The Amazing Spider-Man 3 in 2018.
The studio has also set video game adaptation Uncharted for June 10, 2016 – the slot previously occupied by The Amazing Spider-Man 3.
The action-adventure is based on the PlayStation game series about adventurer Nathan Drake. Seth Gordon will direct and the latest draft of the screenplay is being written by David Guggenheim.
Charles Roven, Avi Arad, Alex Gartner and Ari Arad will produce the Arad/Atlas Entertainment Production.